Partners / INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE

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www.inrae.fr

INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE

UMR-SAVE (Vineyard health and agroecology) of INRA develops research on the wine ecosystem at the interface between ecology, phytopathology and entomology. The aim is to bring out an agroecological protection of the vineyard that makes use of biological regulations, which is based on genetic resistance and biocontrol products and which promotes beneficial associations between plants and microorganisms.

The attractiveness of the “Grapevine Biocontrol” platform is based on the expertise of SAVE and IFV in evaluating the effectiveness of phytosanitary (conventional) as well as biocontrol (micro-organisms or naturally occurring substance) products in the vineyard and in the laboratory, the characterization of their mode of action (antibiosis, antagonist, spatial competition, stimulator of defences) and their integration into

integrated protection strategies or in particular devices.

The laboratory has extensive experience in molecular tools development:

  • a dedicated chip of qPCR to detect and quantify: i)– the presence of resistant downy mildew strains (P. viticola) to fungicides, ii) the presence of strains belonging to genetically distinct groups in powdery mildew populations (strains A and B of E. necator, Dufour et al, 2011) and iii) the presence of powdery mildew resistant to fungicides inhibiting sterol biosynthesis or mitochondrial respiration (DMI and QoI, Dufour et al, 2011).
  • a dedicated qPCR chip to evaluate the level of expression of target genes in response to biotic or abiotic stresses (“BioMolChem” chip, Dufour et al, 2013) by classical real-time PCR techniques (Stratagene Mx3005P system).

Role in the project: INRA is involved in the validation of the biopesticide, in the optimisation of field trials approach and in the evaluation of the product impact on the environment and human health.